Morphological and mathematical analysis of ICSI embryo shapes and developmental fate: preliminary observations.

  • (Aragona C, Galli L, Passaro D, Linari A, Bezerra Espinola MS, Micara G, Tranquilli D, Boninsegna C and Bizzarri M)
  • Proposed for publication

    Abstract: Main and most common methods of embryo analysis and morphological classification currently used in ART medical practice evaluate a multiplicity of morphological parameters, e.g. blastomeres number and symmetry, internal structures homogeneity, presence of corpuscles, cytoplasmatic features and characterization of embryo external shape (regularity, thickness and thickness variation of zona pellucida, ZP). Growing evidences are suggesting that cell shape should be considered as the most critical determinant of embryo competence, therefore quantitative shape descriptors could provide an insight on complex systems being observed. In this study it is presented an original method for calculating a shape form factor (algorithm) linked to local morphological variability of embryo ZP, called Local ZP Variation (LZPV), instead of its macroscopic characteristics. This method is based on harmonic analysis techniques application in frequency-domain representation of ZP inner and outer boundaries, to verify whether harmonic measures could be observationally associated to better results in terms of pregnancy outcome. Preliminary observations show that our method, whether confirmed in a larger series of patients for its validity and reproducibility, may represent a valuable tool for specialists to distinguish embryos of good quality with higher ability to implant and reach term pregnancy.

    SEMIAUTOMATIC ANALYSIS OF DAY 2 ICSI EMBRYO SHAPE VERSUS CLASSICAL EMBRYO RANKING: CLINICAL OUTCOME IN A RETROSPECTIVE CONTROLLED STUDY IN 147 PATIENTS

  • (Aragona C, Linari A, Bezerra Espinola MS, Micara G, Tranquilli D, Boninsegna C and Bizzarri M)
  • Proposed for publication

    Abstract: In the present retrospective controlled study it is considered a semiautomatic method for calculating a shape form factor (algorithm) linked to the local morphological variability of the ZP of ICSI embryo digitalized images. The method is based on the application of harmonic analysis techniques in the frequency-domain representation of the ZP peripheral area, to verify:
    1) whether harmonic measures identifying “Top embryos” could be observationally associated to better results in terms of clinical outcome compared to the “Poor embryos”;
    2) if with any adjunctive contribution to classical I grade embryo scoring “Grade I test”.

    EFSS 17° Annual International Conference, 13-14 December 2012

    The Egyptian Fertility & Sterility Society Cairo - Egypt

    EFSS